One of Ashe's arrows can be seen shot through an apple in the Mac Version Launch of League of Legends.
Her bow is also seen in a later part of the video.
Ashe is the only champion to have exactly 1.0 scaling for both AD and AP on all her abilities with AD/AP ratios; her attack damage and ability power are added without multiplication to the damage output of her Volley and Enchanted Crystal Arrow, respectively.
Several champions like Ezreal have 1.0 AD scaling, but has varying AP scaling, while other champions like Veigar have 1.0 AP scaling but no AD scaling.
Outside the game, Ashe and Tryndamere's relationship is possibly a private Riot reference. Tryndamere is the nickname of their president, Marc Merrill, whose wife's name is Ashley (as revealed in this thread by Merrill).
Ashe and Tryndamere have struck a business partnership with Gragas, selling him ownership of Freljord's Avarosa Iceflow glacier.
He had also, apparently, solved the problem of a unification of a lesser tribe with the Avarosan tribe by getting everyone drunk and drowning their troubles away.
Ashe is in direct conflict with Sejuani, who opposes Ashe's claim as the Queen of Freljord.
Ashe is a distant relative to Sejuani as they both descended from two of the three legendary princesses, who were sisters to one another. Ashe descending from Avarosa while Sejuani from Serylda.